Feb 21 2008
3. Researches
ORAL AND POSTER PRESENTATION
- International Joint Symposium University of Tokushima-Niigata University-Universitas Gadjah Mada, Poster presentation : Comparison of Shear and Microtensile Bond Strength of Composite Repair , December 2010
- IADR conference, Continental European Division (CED), London, UK, Poster presentation : The effect of aging conditions on the repair strength of resin composites, September 2008
- World Biomaterial Congress, Amsterdam, The Netherlands, Poster presentation : Repair Strength of Aged Polymeric Dental Filling Materials, May 2008
- Biomedical, Material Science and Application Meeting in Schiermonikoog, Department of Biomedical Eng, Oral presentation : The effect of aging conditions on the repair strength of resin composite, April 2008
- Biomedical, Material Science and Application Meeting in Schiermonikoog, Department of Biomedical Eng, Oral Presentation : , Effect of various surface conditioning methods on the bond strength of microhybrid and nanofilled composites for immediate repair/relayering, April 2007
- on International Biomedical Engineering Conference, Institute Technology of Bandung, Indonesia, Oral presentation : The effect of various surface treatments on fresh resin-resin bond strength, November 2006
- Faculty of Dentistry Scientific Meeting in Yogyakarta, Gadjah Mada University, Indonesia, Oral presentation : The role of Transforming Growth Factors-Beta on The Tertier Dentine Formation, December 2005
- Lembaga Penelitian, Gadjah Mada University, Recent Progress on Bioceramics, August 2005
- The 13th Scientific Meeting in Jakarta, Indonesia University, Indonesia, Oral presentation: The effect of amniotic membrane on the number of fibroblast cell in gingival wound healing. , October 2002
PUBLICATION
1. Rinastiti, M. Ozcan, Siswomihardjo, and H.J. Busscher, Immediate repair bond strengths of microhybrid, nanohybrid and nanofilled composites after different surface treatments, Journal of Dentistry, vol. 38 / 1, pp. 29-38., 2010
Abstract
OBJECTIVES: To evaluate immediate repair bond strengths and failure types of resin composites with and without surface conditioning and characterize the interacting composite surfaces by their surface composition and roughness.
METHODS: Microhybrid, nanohybrid and nanofilled resin composites were photo-polymerized and assigned to four groups: (1) no conditioning (Control), (2) no conditioning, polymerized against a Mylar strip (Control, with strip), (3) intermediate adhesive resin (IAR) application, and (4) chair-side silica coating, silanization and intermediate resin application (SC). Resin composites, similar as their substrates, were adhered onto the substrates. Shear force was applied to the interface in a universal testing machine and failure types were evaluated under light microscopy. Surface characterization was done by contact angle measurements, X-ray photoelectron spectroscopy, scanning electron and atomic force microscopy.
RESULTS: Significant effects of the resin composite type and surface conditioning were observed. Conditioning the composites with their IARs does not result in significant improvements in bond strength compared to the control with strip (bond strengths between 14.5 and 20.0 MPa). SC increased the bond strength in all composites except TE by an average 8.9 MPa, while in all composites the surface roughness increased from 7 to 384 microm. Failure types in this group were exclusively cohesive. Physico-chemical modelling of the composite surfaces showed that the surfaces were dominated by the resin matrix, with a major increase in silica-coverage after SC for all composites.
CONCLUSION: Intermediate adhesive resin conditioning did not improve the composite-to-composite immediate repair strength. Silica coating and silanization followed by its corresponding IAR, strongly increased repair bond strengths and provided exclusively cohesive failures in the substrate in all composites.
2. H.J. Busscher, Rinastiti, Siswomihardjo, and H.C.v.d. Mei, Biofilm formation on dental restorative and implant materials., Journal of Dental Research, vol. 89(7), pp. 657-65, 2010
Abstract
Biomaterials for the restoration of oral function are prone to biofilm formation, affecting oral health. Oral bacteria adhere to hydrophobic and hydrophilic surfaces, but due to fluctuating shear, little biofilm accumulates on hydrophobic surfaces in vivo. More biofilm accumulates on rough than on smooth surfaces. Oral biofilms mostly consist of multiple bacterial strains, but Candida species are found on acrylic dentures. Biofilms on gold and amalgam in vivo are thick and fully covering, but barely viable. Biofilms on ceramics are thin and highly viable. Biofilms on composites and glass-ionomer cements cause surface deterioration, which enhances biofilm formation again. Residual monomer release from composites influences biofilm growth in vitro, but effects in vivo are less pronounced, probably due to the large volume of saliva into which compounds are released and its continuous refreshment. Similarly, conflicting results have been reported on effects of fluoride release from glass-ionomer cements. Finally, biomaterial-associated infection of implants and devices elsewhere in the body is compared with oral biofilm formation. Biomaterial modifications to discourage biofilm formation on implants and devices are critically discussed for possible applications in dentistry. It is concluded that, for dental applications, antimicrobial coatings killing bacteria upon contact are more promising than antimicrobial-releasing coatings.
3. Rinastiti M, Ozcan M, Siswomihardjo W, Busscher HJ. Effects of surface conditioning on repair bond strengths of non-aged and aged microhybrid, nanohybrid, and nanofilled composite resins. Clin Oral Investig. 2010 May 25. [Epub ahead of print].
Abstract
This study evaluates effects of aging on repair bond strengths of microhybrid, nanohybrid, and nanofilled composite resins and characterizes the interacting surfaces after aging. Disk-shaped composite specimens were assigned to one of three aging conditions: (1) thermocycling (5,000x, 5-55 degrees C), (2) storage in water at 37 degrees C for 6 months, or (3) immersion in citric acid at 37 degrees C, pH 3 for 1 week; a non-aged group acted as the control. Two surface conditionings were selected: intermediate adhesive resin application (IAR-application) and chairside silica coating followed by silanization and its specific IAR-application (SC-application). Composite resins, of the same kind as their substrate, were adhered onto the substrates, and repair shear bond strengths were determined, followed by failure type evaluation. Filler particle exposure was determined by X-ray photoelectron spectroscopy and surface roughness analyzed using scanning electron and atomic force microscopy. Surface roughness increased in all composite resins after aging, but filler particle exposure at the surface only increased after thermocycling and citric acid immersion. Composite resin type, surface conditioning, and aging method significantly influenced the repair bond strengths (p < 0.05, three-way analysis of variance) with the least severe effects of water storage. Repair bond strengths in aged composite resins after IAR-application were always lower in non-aged ones, while SC-application led to higher bond strengths than IAR-application after thermocycling and water storage. In addition, SC-application led to more cohesive failures than after IAR-application, regardless the aging method.
4. Rinastiti M, Ozcan M, Siswomihardjo W, Busscher HJ. Immediate repair bond strengths of microhybrid, nanohybrid and nanofilled composites after different surface treatments. J Dent. 2010 Jan;38(1):29-38. Epub .
Abstract
OBJECTIVES: To evaluate immediate repair bond strengths and failure types of resin composites with and without surface conditioning and characterize the interacting composite surfaces by their surface composition and roughness. METHODS: Microhybrid, nanohybrid and nanofilled resin composites were photo-polymerized and assigned to four groups: (1) no conditioning (Control), (2) no conditioning, polymerized against a Mylar strip (Control, with strip), (3) intermediate adhesive resin (IAR) application, and (4) chair-side silica coating, silanization and intermediate resin application (SC). Resin composites, similar as their substrates, were adhered onto the substrates. Shear force was applied to the interface in a universal testing machine and failure types were evaluated under light microscopy. Surface characterization was done by contact angle measurements, X-ray photoelectron spectroscopy, scanning electron and atomic force microscopy. RESULTS: Significant effects of the resin composite type and surface conditioning were observed. Conditioning the composites with their IARs does not result in significant improvements in bond strength compared to the control with strip (bond strengths between 14.5 and 20.0 MPa). SC increased the bond strength in all composites except TE by an average 8.9 MPa, while in all composites the surface roughness increased from 7 to 384 microm. Failure types in this group were exclusively cohesive. Physico-chemical modelling of the composite surfaces showed that the surfaces were dominated by the resin matrix, with a major increase in silica-coverage after SC for all composites. CONCLUSION: Intermediate adhesive resin conditioning did not improve the composite-to-composite immediate repair strength. Silica coating and silanization followed by its corresponding IAR, strongly increased repair bond strengths and provided exclusively cohesive failures in the substrate in all composites.
5. Rinastiti M, Harijadi, A.l. Supartinah, W. Sosroseno. Histological evaluation of rabbit gingival wound healing transplanted with human amniotic membrane. International Journal of Oral Maxillofacial Surgery. 2006 ; 35 : 247-251.
Abstract
Human amniotic membrane has been used as a material to accelerate wound healing and reconstruct damaged organs. The aim of the present study was to assess histologically human amniotic membrane transplantation on rabbit’s gingival wound. Three- to 4-month-old male rabbits were divided into 2 groups, i.e., control (group I) and amniotic membrane-transplanted animals (group II). Buccal gingival wounds were created by a punch-biopsy instrument and covered by a 5-layered human amniotic membrane for group II or left uncovered for group I. Gingival biopsies were taken at days 1, 3, 5, 7 and 10, processed for paraffin sections and stained with haematoxylin-eosin or von Gieson. Thickness of epithelial layer, the number of polymorphonuclear cells (PMN), fibroblasts and new blood vessels as well as density of collagen fibres were assessed. The results showed that the number of fibroblasts and new blood vessels, but not PMN, from group II was higher than that from group I (P < 0.05). Similarly, the epithelial thickness and density of collagen fibres from group II were significantly higher than those from group I (P < 0.05). The results of the present study indicate that amniotic membrane transplantation may induce rapid epithelialization and both granulation tissue and collagen formation but suppress inflammation, suggesting that amniotic membrane transplantation may promote rapid gingival wound healing in rabbits compared to secondary healing.
Leave a Reply
You must be logged in to post a comment.